Protein Engineering Handbook

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In three topical volumes, it covers the full spectrum of current concepts, methods and application areas.

VOLUME 1: FUNDAMENTALS
Historical perspective
Structure and function in proteins
Protein engineering and dynamics
Evolution: Protein engineering in Nature
Metagenomics and genome prospection
Engineering the reaction environment
Patents and legal aspects

VOLUME 2: METHODS
In silico protein design
Rational protein engineering
Protein evolution in the laboratory
Selection and screening
In situ directed evolution
Library evaluation
Production scale biosynthesis

VOLUME 3: APPLICATIONS
Antibody engineering
Engineering secondary metabolism
Pathway engineering and multienzyme systems
Engineering thermostability
Novel enzyme functions
Engineering substrate specificity
Introducing unnatural amino acids
Glycosylation engineering
Inteins in protein engineering
Biosensors and regulators
Protein folding
Plant proteins
Biomaterials


Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering.

Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Technical Chemistry and Biotechnology at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.