Handbook of Biochemistry and Molecular Biology

Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fourth edition of the Handbook of Biochemistry and Molecular Biology represents a dramatic revision — the first in two decades — of one of biochemistry's most referenced works. This edition gathers a wealth of information not easily obtained, including information not found on the web.

Offering a molecular perspective not available 20 years ago, it provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents.

Just a small sampling of the wealth of information found inside the handbook:

Buffers and buffer solutions
Heat capacities and combustion levels
Reagents for the chemical modification of proteins
Comprehensive classification system for lipids
Biological characteristics of vitamins
A huge variety of UV data
Recommendations for nomenclature and tables in biochemical thermodynamics
Guidelines for NMR measurements for determination of high and low pka values
Viscosity and density tables
Chemical and physical properties of various commercial plastics
Generic source-based nomenclature for polymers
Therapeutic enzymes
About the Editors:

Roger L. Lundblad, Ph.D.

Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD degree in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at the Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently Dr. Lundblad is an independent consultant and writer in biotechnology in Chapel Hill, North Carolina. He is an adjunct Professor of Pathology at the University of North Carolina at Chapel Hill and Editor-in-Chief of the Internet Journal of Genomics and Proteomics.

Fiona M. Macdonald, Ph.D., F.R.S.C.

Fiona M. Macdonald received her BSc in chemistry from Durham University, UK. She obtained her PhD in inorganic biochemistry at Birkbeck College, University of London, studying under Peter Sadler. Having spent most of her career in scientific publishing, she is now at Taylor and Francis and is involved in developing chemical information products.


Amino Acids, Peptides and Proteins


Properties of Amino Acids

Data on the Naturally Occurring Amino Acids

Structures and Symbols for Synthetic Amino Acids Incorporated Into Synthetic Polypeptides

Unnatural Amino Acids for Incorporation into Proteins

Properties of the α-Keto Acid Analogs of Amino Acids

α,β-Unsaturated Amino Acids

Amino Acid Antagonists

Coefficients of Solubility Equations of Certain Amino Acids in Water

Heat Capacities, Absolute Entropies, and Entropies of Formation of Amino Acids and Related Compounds

Heat of Combustion, Enthalpy and Free Energy of Formation of Amino Acids and Related Compounds

Solubilities of Amino Acids in Water at Various Temperatures

Heats of Solution of Amino Acids in Aqueous Solution at 25°C

Free Energies of Solution and Standard Free Energy of Formation of Amino Acids in Aqueous Solution at 25°C

Far Ultraviolet Absorption Spectra of Amino Acids

UV Absorption Characteristics of N-Acetyl Methyl Esters of the Aromatic Amino Acids, Cystine and of N-Acetylcysteine

Numerical Values of the Absorbances of the Aromatic Amino Acids in Acid, Neutral and Alkaline Solutions

Luminescence of the Aromatic Amino Acids

Luminescence of Derivatives of the Aromatic Amino Acids

Luminescence of Proteins Lacking Tryptophan

Luminescence of Proteins Containing Tryptophan

Hydrophobicities of Amino Acids and Proteins

Chemical Specificity of Reagents for Protein Modification

Reagents for the Chemical Modification of Proteins

Protein pK Values

Protease Inhibitors and Protease Inhibitor Cocktails

Assay of Solution Protein Concentration

Spectrophotometric Determination of Protein Concentration in the Short-Wavelength Ultraviolet

Lipids


A Comprehensive Classification System for Lipids

Properties of Fatty Acids and Their Methyl Esters

Densities, Specific Volumes, and Temperature Coefficients of Fatty Acids from C8 to C12

Composition and Properties of Common Oils and Fats

Androgens

Bile Acids

Corticoids

Estrogens

Progestogens

Sterols

Prostaglandins and Related Fatty-Acid Derived Materials

Vitamins and Coenzymes

Properties of Vitamins

Biological Characteristics of Vitamins

Properties for Ascorbic Acid and Ascorbate-2-Sulfate

Vitamers

Vitamin Names Discarded

Nucleic Acids


UV Spectral Characteristics and Acidic Dissociation Constants of 280 Alkyl Bases, Nucleosides, and Nucleotides

Ultraviolet Absorbance of Oligonucleotides Containing 2′-O-Methylpentose Residues

Spectrophotometric Constants of Ribonucleotides

Purines, Pyrimidines, Nucleosides, and Nucleotides: Physical Constants and Spectral Properties

Chemical Modification of Nucleic Acids

Transfection Technologies

Carbohydrates


Introduction to Carbohydrates

Natural Alditols, Inositols, Inososes, and Amino Alditols and Inosamines

Natural Acids of Carbohydrate Derivation

Natural Aldoses

Natural Ketoses

Carbohydrate Phosphate Esters

The Naturally Occurring Amino Sugars

Oligosaccharides (Including Disaccharides)

Mucopolysaccharides (Glycosaminoglycans)

Physical and Chemical Data

Recommendations for Nomenclature and Tables in Biochemical Thermodynamics

Standard Transformed Gibbs Energies of Formation for Biochemical Reactants

Enthalpy, Entropy, and Free Energy Values for Biochemical Redox Reactions

Oxidation-Reduction Potentials, Absorbance Bands and Molar Absorbance of Compounds used in Biochemical Studies

Calorimetric ΔH Values Accompanying Conformational Changes of Macromolecules in Solution

Free Energies of Hydrolysis and Decarboxylation

Deci-Normal Solutions of Oxidation and Reduction Reagents

Guidelines for Potentiometric Measurements in Suspensions Part A. The Suspension Effect

Ionization Constants of Acids and Bases

Guidelines for NMR Measurements for Determination of High and Low pKa Values

Measurement and Interpretation of Electrokinetic Phenomena

Measurement of pH Definition, Standards, and Procedures

General Comments on Buffers

List of Buffers

Bronsted Acidities

Measurement of pH

Buffer Solutions

Amine Buffers Useful for Biological Research

Preparation of Buffers for Use in Enzyme Studies

Buffer for Acrylamide Gels (Single-Gel Systems)

Starch Gels

Indicators for Volumetric Work and pH Determinations

Acid and Base Indicators

Specific Gravity of Liquids

Viscosity and Density Tables

A Listing of Log P Values, Water Solubility, and Molecular Weight for Some Selected Chemicals

Chemicals Commonly Used in Biochemistry and Molecular Biology and Their Properties

Common Detergents Used in Biochemical Research

Some Properties of Detergents and Surfactants Used in Biochemistry and Molecular Biology

Some Biological Stains and Dyes

Mordant Dyes

Metal Chelating Agents

Water

Stability of Solutions for GLP and cGMP Use

General Information on Spectroscopy

Microplates

Plastics

Chemical and Physical Properties of Various Commercial Plastics

Generic Source-Based Nomenclature for Polymers

Definitions of Terms Relating to Reactions of Polymers and to Functional Polymeric Materials

Definitions of Terms Related to Polymer Blends, Composites, and Multiphase Polymeric Materials

Organic Name Reactions Useful in Biochemistry and Molecular Biology

Enzymes in Synthetic Organic Chemistry

Therapeutic Enzymes

Weights of Cells and Cell Constituents

Particle Diameter

Appendix A: Abbreviations and Acronyms

Appendix B: Glossary of Terms Useful in Biochemistry

Index